Development and Validation of an Analytical Method for β-Agonists in Livestock and Fishery Products Using LC-MS/MS

• DOI: 10.5338/kjea.2022.41.2.17
• Publication Date: 2022-07-01T07:27:21Z
• Authors: Tae Ho Lee; Yu Ra Kim; Su Jeong Park; Ji Young Kim; Jang Duck Choi; Gui Im Moon
• Keywords: 0402 animal and dairy science; 04 agricultural and veterinary sciences

BACKGROUND:The β-agonists known as phenyl ethanolamine derivatives have a conjugated aromatic ring with amino group.They are used tocolytic agents and bronchodilator to human animal generally, some of them growth promoters livestock.METHODS AND RESULTS: in samples were extracted by 0.4 N perchloric acid ethyl acetate.The target compounds analyzed liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS).Validation method was performed according CODEX guidelines (CAC/GL-71).The matrix matched calibration gave correlation coefficients>0.98,and the obtained recoveries range 62.0-109.8%,with relative standard deviation ≤ 20.1%.In addition, survey inspect any residual β-agonist from 100 livestock fishery products ractopamine detected one samples.CONCLUSION(S): In this study, we established analytical for through using expanded samples.And anticipate that would be analysis determine veterinary drug residues products.