An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals

• DOI: 10.5455/ajvs.226337
• Publication Date: 2025-01-02T10:23:52Z
• Authors: Asmaa Eldeen; Rania Rania; S M; Abdelhalim Elshaw...; El Gedawy; A A
• Keywords: Subspecies

Mycobacterium avium subspecies paratuberculosis(MAP) is one of the most infectious bacteria affecting small and large ruminants causing Johne's disease which characterized by chronic diarrhea , severe emaciation decreasing milk production leading to highly effective economic losses. Also, MAP causes in human. In this study, blood without anticoagulant (n=200) faecal samples were collected from sheep, goats, cattle buffaloes. By using ZN stain, smears decontaminated revealed presence acid fast bacilli (68%). Serum separated tested ELISA showed antibodies against sheep (20%), goats (17.1%), (22.5%), buffaloes (16%). Faecal PCR for detection IS900 specific gene confirmation strains it was detected (26.7%) (25.7%) (28.8%) cattle, (20%) conventional used differentiate between 2 types where S- type (9)sheep, (5) (12) (3) C- (7) (4) (11) (2) This study proved that a widespread bacterium shows spreading its ruminants.