Genetic diversity and population structure of Echinococcus multilocularis: An in-silico global analysis

• DOI: 10.5455/javar.2024.k772
• Publication Date: 2024-07-14T09:30:55Z
• Authors: Ayed Alshammari; Muhammad Subhani; Majed Wakid; Abdulsalam Alkhaldi; Shujaat Hussain; Muhammad Malik; Muhammad Saqib; Warda Qamar; Mughees Alvi
• Keywords: Nucleotide diversity; Echinococcus multilocularis; Mega-

Objectives: Alveolar echinococcosis is caused by Echinococcus multilocularis, a parasite of zoo¬notic significance with wide range intermediate and final hosts, the survives suc¬cessfully in diversified conditions. Plentiful studies have been done to study genetic structure population level intimate kinship using mitochondrial (mt) DNA. The present was conducted investigate structure, variation, phylogenetic relationship various isolates E. multiocularis submitted GenBank worldwide. Sequences mt genes (mt-cytochrome c oxidase (cox1), mt-NADH dehydrogenase (nad1)) multilocularis were analyzed achieve set goals. Materials Methods: A total 275 124 gene sequences mt-cox1 mt-nad1 belong¬ing respectively, retrieved from National Center for Biotechnology Information GenBank. subjected alignment respective reference MEGA software. PopArt software used establish medi¬an-joining networks, while DnaSp calculate neutrality diversity indices. MrBayes association between haplotypes based on Bayesian phylogeny. Results: Approximately 13 20 distinctive nad1 cox1 genes, observed study. In both indices indicated low haplo¬type (mt-cox1 = 0.140; 0.374) nucleotide 0.00111; 0.00287) diversities. values Tajima’s D Fu Fs under found be negative. Conclusion: This maiden attempt provide insights into variation global scale. However, it suggested that better understand more geographical locations amplifications full-length should considered, which could helpful widening multilocularis.