Detection of rare point mutation via allele-specific amplification in emulsion PCR

• DOI: 10.5483/bmbrep.2013.46.5.155
• Publication Date: 2013-07-01T05:48:38Z
• Authors: Changming Cheng; Yin Zhou; Chao Yang; Juan Chen; Jie Wang; Jie Zhang; Guoping Zhao
• Keywords: Amplicon

It is essential to analyze rare mutations in many fields of biomedical research. However, the detection usually failed due interference predominant wild-type DNA surrounded. Herein we describe a sensitive and facile method detecting point mutation on basis allele-specific amplification emulsion PCR. The identification selective are accomplished one-pot reaction. primers coupled magnetic beads allow exclusive enrichment mutant amplicons. productive bearing amplicons subsequently stained with fluorescent dyes. Thus, percentage as low 0.1%, can be detected by analysis. relative percentages among different samples roughly accessed counting fraction positive through flow cytometry.