Genotoxicity Study of Magnolia obovata Extracts

• DOI: 10.5487/tr.2007.23.1.073
• Publication Date: 2011-05-23T00:10:20Z
• Authors: Seung-Ho Lee; Jae-Myun Ryu; Im-Kwon Seo; Tae-Hee Lee; Yun-Bae Kim; Sung-Kwon Moon; Kyung-Hwan Jung; Ki-Rang Park; Seock-Yeon Hwang
• Keywords: Chromosome aberration; Chinese hamster; Magnoliaceae; Ames test

To evaluate the immuno-toxicity of magnolia extracts, mutagenicity of Salmonella, chromosome aberration of Chinese hamster ovary (CHO) cells and micronucleus formation in rats were examined. Magnolia extracts at the concentrations of 312~5,000 μg/plate did not induce mutagenicity in Salmonella typhimurium TA 98, TA 100 and TA 1535 with and without metabolic activation of S-9 mixture. In chromosome aberration assay, Magnolia extracts at the concentrations of 50~800 μg/plate did not cause a significant chromosome aberration in CHO cells with and without metabolic activation of S-9 mixture. Magnolia extracts were treated with dose of 0.5, 1 and 2 g/㎏ in ICR mice. After 48 hours, the frequencies of the micro-nucleided polychromasia erythrocytes (MNPCE) were determined in bone marrows isolated from the mice. Magnolia extracts did not increase the incidence of polychromasia erythrocytes of bone marrow in ICR mice. These results show that Mgnolia extracts did not induce any harmful genotoxic effects.