Study on the Mechanism of Aerobic Exercise Alleviating Osteoporosis in Ovariectomized Mice through Regulating the RANKL/RANK/OPG Pathway

• DOI: 10.56028/aetr.13.1.571.2025
• Publication Date: 2025-04-07T06:03:31Z
• Authors: Dan Wang; Juan Bao; Pei Wu; Cailian Ruan
• Keywords: Aerobic Exercise; Rank (graph theory)

Objective This study aimed to explore the regulatory effects of aerobic exercise on RANKL/RANK/OPG signaling pathway and evaluate its potential therapeutic osteoporosis in ovariectomized mice (OVX).Methods:1.Animal Experiment: 40 2-month-old SPF male SD rats, weighing (225±25) g, were purchased from Animal Experiment Center Xi’an Jiaotong University (Qualification Certificate No.: Shaanxi Medical Practice No. 17). The experiment was conducted at Experimental Yan’an College. Rats had free access food water, circadian rhythm 12 h. experimental process strictly followed Provincial Regulations Administration Laboratory Animals.An mouse model established. Histomorphological Assessment: HE staining used assess morphological impact osteoclasts, TRAP bone resorption pit assay effect osteoclast function. Molecular Biological Analysis: qRT-PCR Western blotting employed investigate regulation by expression RANKL, RANK, IκB, NF-κB, p-IκB proteins their mRNA levels tissue.2. Cell After 8 weeks intervention, sacrificed, macrophage precursor cells extracted forelimbs’ femurs for experiments, including: Activity CCK-8 observe RANKL-induced activity.Biochemical Detection: serum calcium, ALP, BALP, PTH, phosphate, calcitonin evaluated. Results 1. results showed that number osteoclasts per surface (N.Oc/BS) group significantly lower than (p <0.001). 2. Serum Biochemical Compared with group, showed: Assay: activity DMSO reduced <0.01, p <0.001).Serum Levels: PTH decreased <0.01), while phosphate increased <0.01).qRT-PCR Blotting: E2 OPG increased, protein TRAP, Cathepsin K NFATc1, c-Fos, ATP6V0D2, CTSK, DC-STAMP, MMP9 <0.01 or Aerobic inhibited IκB phosphorylation (p<0.01).Conclusion:Aerobic inhibits regulating pathway, thereby improving mice. finding providestheoretical support clinical guidelines treatment postmenopausal women.