Bacterial Communities from the Arsenic Mine in Złoty Stok, Sudety Mountains, Poland

• DOI: 10.5604/01.3001.0010.4875
• Publication Date: 2017-09-28T07:42:27Z
• Authors: TOMASZ CŁAPA; DOROTA NAROŻNASIUDA; RAFAŁ SIUDA; ANDRZEJ BORKOWSKI; MAREK SELWET; CEZARY J. MĄDRZAK; EWA KOŹLECKA
• Keywords: DNA, Bacterial; 0301 basic medicine; Geologic Sediments; Bacteria; Microbiota; QH426-470; Microbiology; Polymerase Chain Reaction; QR1-502; Mining; 6. Clean water; supergene minerals; Arsenic; 03 medical and health sciences; metagenomic approach; RNA, Ribosomal, 16S; Genetics; Poland; microbial community; arsenic mine ecosystem; Polymorphism, Restriction Fragment Length

Abstract Investigations of bacterial communities and characterization mineralogy the environment in Złoty Stok As-Au deposit were carried out. PXRD analysis revealed presence picropharmacolite as most common secondary arsenic mineral mine. Total DNA was extracted from slime streams or biofilms samples to investigate communities. PCR amplification 16S rDNA performed followed by subcloning its products. Over 170 clones analyzed means RFLP method. Eight group representing different restriction patterns identified. The nucleotide sequences their inserts suggest that bacteria present mine belong to: Flavobacteria , Sphingobacteriia Bacteroides Proteobacteria Mollicutes Firmicutes . metagenomic approach allows demonstrate a higher diversity microbiota than classical microbiological studies cultivable isolates.