Microbiological analysis of tracheostomy tube biofilms and antibiotic resistance profiles of potentially pathogenic microorganisms

• DOI: 10.5604/01.3001.0015.8827
• Publication Date: 2023-01-05T08:48:09Z
• Authors: Łukasz Ścibik; Dorota Ochońska; Monika Golda-Cepa; Monika Brzychczy-...; Andrzej Kotarba
• Keywords: Adult; Staphylococcus aureus; 03 medical and health sciences; Tracheostomy; 0302 clinical medicine; Drug Resistance, Multiple, Bacterial; Biofilms; Gram-Negative Bacteria; Humans; 3. Good health

<b>Introduction:</b> In hospitalized patients, tracheostomy tubes (TTs) are susceptible to colonization by biofilm- producing potentially pathogenic microorganisms (PPMs). Contact with TTs, which situated in a critical region of the body enormous microbial exposure, may lead emer-gence resistant respiratory infections.</br></br> <b>Objective:</b> Our study aimed isolate and identify Gram-positive Gram-negative PPMs, mark their antibiotic resistance determine bacteriological pattern biofilm colonizing TTs. </br></br> <b>Methods:</b> The was conducted on 45 obtained from adult patients intubation periods ranging 1 28 days. Tracheal aspirates (TA) polyvinyl chloride (PVC) TTs were used for analysis. Bacteria biofilms identified standard microbiological techniques, tested phenotypic according EUCAST guidelines visualized SEM.</br></br> <b>Results:</b> Out 100% found be positive bacterial cultures 58 PPM isolates (10 spe-cies) correlating well SEM findings. Overall, 72% bacilli, followed cocci (28%). Staphylococcus aureus predominant bacterium (identified 35.5% patients), Klebsiella pneumoniae 23.8%). Among 50% as multidrug-resistant (MDR), 8.6% extremely drug-resistant (XDR) 5.2% pandrug-resistant (PDR).</br></br><b>Conclusions:</b> showed rapid TT surface PPMs. Patients tracheosto- mies, also those non-infectious conditions, mainly colonized highly re-sistant bacteria.