Mitochondrial Dysfunction of Immortalized Human Adipose Tissue-Derived Mesenchymal Stromal Cells from Patients with Parkinson’s Disease

• DOI: 10.5607/en.2013.22.4.283
• Publication Date: 2014-01-18T09:22:41Z
• Authors: Hyo Eun Moon; Seung Hee Yoon; Yong Suk Hur; Hyung Woo Park; Ji Young Ha; Kyung-Hee Kim; Jung Hee Shim; Seung Hyun Yoo; Jin H. Son; Seung Leal Paek; In Keyoung Kim; Jae Ha Hwang; Dong Gyu Kim; Han-Joon Kim; Beom Seok Jeon; Sung Sup Park; Sun Ha Paek
• Keywords: 0301 basic medicine; 03 medical and health sciences; Original Article

Mitochondrial dysfunction in dopaminergic neurons of patients with idiopathic and familial Parkinson's disease (PD) is well known although the underlying mechanism not clear. We established a homogeneous population human adipose tissue-derived mesenchymal stromal cells (hAD-MSCs) from adult early-onset hereditary Parkin-defect PD as late-onset by immortalizing hTERT gene to better understand PD. The hAD-MSCs were designated "PD", "Parkin" pituitary adenomas "non-PD" short. pGRN145 plasmid containing was introduced establish telomerase immortalized cells. hTERT-immortalized cell lines showed chromosomal aneuploidy sustained stably over two-years. morphological study mitochondria primary that non-PD normal; however, those Parkin gradually damaged. A striking decrease mitochondrial complex I, II, IV activities observed Comparative Western blot analyses performed investigate expressions specific marker proteins lines. This suggests hAD-MSC could be good cellular models evaluate pathogenesis develop early diagnostic markers effective therapy targets for treatment