Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay

• DOI: 10.5808/gi.21048
• Publication Date: 2021-09-30T05:45:08Z
• Authors: Jiyon Chu; Juyoun Shin; Shinseok Kang; Sun Shin; Yeun-Jun Chung
• Keywords: Recombinase Polymerase Amplification

Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive detection of species. We designed LAMP primers targeting hilA gene as universal marker A total seven strains 11 non-Salmonella pathogen from eight different genera were used in study. All showed positive signals with assay; however, there was no non-specific signal strains. The limit 100 femtograms (20 copies per reaction), which ~1,000 times more than limits conventional polymerase chain reaction (PCR) (100 pg). time less 20 minutes, one-third taken while using PCR. conclusion, our accurately detected higher degree sensitivity greater rapidity PCR assay, it may be suitable point-of-care testing field.