Optimization of Submerged Fermentation Medium for Matrine Production by Aspergillus terreus, an Endophytic Fungus Harboring Seeds of Sophora flavescens, Using Response Surface Methodology

• DOI: 10.5941/myco.2017.45.2.90
• Publication Date: 2017-08-02T01:15:40Z
• Authors: Qiang Zhang; Yujuan Li; Fangxue Xu; Mengmeng Zheng; Xiaozhi Xi; Xuelan Zhang; Chunchao Han
• Keywords: Sophora flavescens; Aspergillus terreus; Endophyte; Sophora

Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied optimize medium components endophytic fungus. Results indicated that endophyte Aspergillus terreus had matrine. The single factor tests demonstrated potato starch best carbon source and combination peptone NH4NO3 optimal nitrogen A. terreus. model RSM predicted gain maximal production at 20.67 μg/L, when 160.68 g/L, 24.96 g/L 2.11 g/L. When cultured in medium, yield an average 20.63 ± 0.11 which consistent with prediction. This study offered alternative by fungus fermentation may have far-reaching prospect value.