Isolation and Characterization of Lytic Pseudomonas fluorescens Bacteriophage isolated from milk

• DOI: 10.59796/jcst.v13n2.2023.885
• Publication Date: 2023-07-18T03:48:35Z
• Authors: Rinratree Wongyoo; Sirinthorn Suntho...; Komwit Surachat; Thassanant Atithep; Achariya Rangsiruji; Siriruk Sarawanee...; Onanong Pringsulaka
• Keywords: Pseudomonas fluorescens; Lytic cycle; Myoviridae; Multiplicity of infection; Isolation

Phage-based biocontrol is an alternative method for preventing and controlling the occurrence of Pseudomonas spp. in food products. However, use bacteriophages to control heat-stable protease fluorescens still rare. The objectives this study were isolate lytic P. fluorescens, evaluate their efficacy at laboratory level. Among these strains, only one phage, ΦTIS1, was isolated by using TISTR 1887 as host. Further analysis electron microscopy indicated that ΦTIS1 belongs Myoviridae family. This phage relatively stable pH temperature ranges 4.0 12.0 4, 30, 37, 45°C, respectively, after 1.5 h incubation. partial genome a linear double-stranded with total length 87,646 bp G + C content 54.71%. In vitro studies effect against various multiplicity infection (MOI) values showed significant decrease bacterial numbers during 6-12 hours incubation, followed regrowth. able significantly reduce compared without phage. These findings suggest has potential be effective prevalence spoilage-causing strains dairy industries.