Interaction Analysis Between Polyglutamine-expanded TATA Box Binding Protein (TBP) and High Mobility Group Box 1 (HMGB1)

• DOI: 10.6248/bf.2013.48.03
• Publication Date: 2013-06-01
• Authors: Shuting Yang; Hsiang-Ming Chen; Li‐Ching Lee; Shih-Han Huang; Chih‐Hsin Lin; Guan‐Chiun Lee; Cheng‐Kang Lee; Guey‐Jen Lee‐Chen
• Keywords: TATA-binding protein; HMGB1; TATA-Box Binding Protein; TATA box; Immunoprecipitation; High-mobility group

Spinocerebellar ataxia type 17 (SCA17) involves the expression of a polyglutamine-expanded TATA box binding protein (TBP), general transcription initiation factor. TBP interacts with other factors, including high mobility group 1 (HMGB1), to regulate gene expression. In this study, we examined effect Q-tract length on HMGB1 using recombinant and N-terminal TBP/Q20~61 proteins. The intracellular association was first confirmed by half-in vivo co-immunoprecipitation (co-IP) glutathione S-transferase (GST) pull-down assays in co-expressed HEK-293 cells. GST assay soluble proteins expressed E. coli also revealed activity between TBP. When strength explored real time quartz crystal microbalance (QCM) assay, length-dependent decrease HMGB1-TBP interaction demonstrated. Together reported increase rate stability TBP/TATA interaction, our study results suggest that polyQ-expanded may contribute pathogenesis SCA17.