Isolation, identification and mutants generation of isolate 04 for its improved bioconversion of ferulic acid to vanillin

• DOI: 10.71336/jabs.1381
• Publication Date: 2025-01-31T17:22:44Z
• Authors: Nagaraju Bathini; Vishnuvardhan Red...; Thirumala Mothe
• Keywords: Bioconversion; Biotransformation

Vanillin is widely used as a food flavouring, scent ingredient in cosmetics and perfumes, an intermediate agrochemicals & medicines also nutraceutical. Serial dilution of sediment sample yielded 32 strains, among which three isolates A2, A7, 04 reported here were characterized at molecular level by 16S rDNA sequencing. They identified similar to Pseudomonas aeruginosa (94.97%), Acinetobacter baumannii (99.93%) Lysinibacillus macroides (98.67%) respectively based on its sequences. Among these isolate was with high ferulic acid (FA) vanillin bioconversion activity. concentration determined the Thiobarbituric (TBA) method analysed UV-spectrophotometry, showed 11.63 ± 0.05 mg/ml after optimization studies. HPLC UV detector for characterization formed, peak 8.793 rt. To improve FA bioconversion, physical mutagenesis performed 04, generated mutants (1, 2, 3). These subject biotransformation study. TBA spectrophotometric analysis higher conversion mutant 2 than other two mutants, 1 3. Mutant formed almost two-fold i.e. 19.78 when compared wild strain, 04. Hence, this may be further subjected studies exploited industrial process.