Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres

• DOI: 10.7287/peerj.preprints.2033v1
• Publication Date: 2018-01-12T20:19:59Z
• Authors: Patricia P Garcez; Juliana Minardi N...; Janaina Mota de V...; Rodrigo Madeiro d...; Rodrigo Delvecchio; Pablo Trindade; Erick Correia Loiola; Luiza M. Higa; Juliana Cassoli; Gabriela Vitória; Patricia Sequeira; Jaroslaw Sochacki; Renato S. Aguiar; Hellen Thais Fuzii; Ana M. Bispo de F...; João Lídio da Sil...; Daniel Martins-de...; Amilcar Tanuri; Stevens K Rehen

Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular and cellular consequences of Zika virus circulating in Brazil to the human brain development have not been studied yet. Here we describe alterations in human neurospheres derived from neural stem cells infected with Brazilian ZIKV. Combined proteomics and mRNA transcriptional profile analyses showed that Brazilian ZIKV, prior to induce cell death, alters cell cycle and halts neurogenic programmes, in addition to regulate transcription and protein translation due to viral replication. These results point to biological mechanisms potentially implicated in brain malformations as a result of ZIKV congenital infection.