Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

• DOI: 10.7314/apjcp.2012.13.12.6109
• Publication Date: 2013-05-15T01:08:37Z
• Authors: Khor Goot-Heah; Thong Kwai-Lin; Gabriele Ruth Ani...; Mannil Thomas Abr...; Nik Mohd Mazuan N...; Rosnah Binti Zain
• Keywords: 03 medical and health sciences; 0302 clinical medicine; DNA, Viral; Papillomavirus Infections; Humans; Mouth Neoplasms; Saliva; Papillomaviridae; 3. Good health

Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is risk factors for developing oral cancer. For this study HPV18 was chosen as it high HPV types may lead to carcinogenesis. However, prevalence infection in Squamous Cell Carcinoma Malaysia remains unclear. Objective: This aimed investigate viral load DNA OSCC potentially malignant lesions using saliva samples. Materials Methods: Genomic DNAs thirty samples normal subjects compromised 16 from 14 patients were amplified a nested polymerase chain reaction analysis. All PCR products then analyzed Bioanalyzer confirm presence DNA. Result: From examined, only 30 (3.3%) cases found be positive study. Conclusion: The finding revealed that there low detection Malaysian by samples, suggesting not important group OSCC.