Comparative Proteomics Analysis of Colorectal Cancer

• DOI: 10.7314/apjcp.2012.13.4.1663
• Publication Date: 2012-09-07T00:33:01Z
• Authors: Jun-Jiang Wang; Ying Liu; Yang Zheng; Feng Lin; Guan-Fu Cai; Xue-Qing Yao
• Keywords: Annexin A2; Annexin A1; Immobilized pH gradient; Pathogenesis

Protein expression in colon and rectal cancer (CRC) paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins.Five fresh colorectal adjacent were obtained protein spots determined using PDQuest software, with identification on the basis of MALDI- TOF mass spectra.Compared mucosa, abnormal 65 varying more than 1.5 times found 2-DE gels from samples (P<0.05); forty-two proteins up-regulated 23 down-regulated; twelve identified spectrometry, which 8 up-regulated, including HSPB1 Annexin A4, while 4 down-regulated, results being consistent Western blot findings.Two-dimensional reference maps for CRC mucosa (NMC) established 12 identified. Up-regulated A4 may play many important roles pathogenesis cancer.