(-)-Epigallocatechin-3-Gallate Induces Apoptosis and Inhibits Invasion and Migration of Human Cervical Cancer Cells

• DOI: 10.7314/apjcp.2012.13.9.4815
• Publication Date: 2013-02-06T07:03:16Z
• Authors: Chhavi Sharma; Qurrat El-Ain Nusri; Salema Begum; Elham Javed; Tahir A. Rizvi; Arif Hussain
• Keywords: HeLa; DNA laddering; Viability assay; Gentamicin protection assay

Invasion and metastasis are the major causes of cancer-related death. Pharmacological or therapeutic interventions such as chemoprevention progression stages neoplastic development could result in substantial reduction incidence cancer mortality. (-)-Epigallocatechin-3-gallate (EGCG), a promising chemopreventive agent, has attracted extensive interest for therapy utilizing its antioxidant, anti- proliferative inhibitory effects on angiogenesis tumor cell invasion. In this study, we assessed influence EGCG potential HeLa cells by viability assay authenticated results nuclear morphological examination, DNA laddering cycle analysis. Further analyzed anti-invasive properties wound migration gene expression MMP-9 TIMP-1 cells. Our indicated that induced growth inhibition dose- time- dependent manner. It was observed death mediated through apoptosis. Interestingly, effectively inhibited invasion modulated related genes (MMP-9 TIMP-1) . These indicate may suppress promotion cervical development.