Screening of MicroRNA in Patients with Esophageal Cancer at Same Tumor Node Metastasis Stage with Different Prognoses

• DOI: 10.7314/apjcp.2013.14.1.139
• Publication Date: 2013-06-18T05:46:27Z
• Authors: Bao-Sheng Zhao; Shang-Guo Liu; Tian-Yun Wang; Ying-Hua Ji; Bo Qi; Yi-Peng Tao; Han-Chen Li; Xiang-Nan Wu
• Keywords: Distant metastasis

Patients at the same pathological stage of esophageal cancer (EC) that received surgical therapy by surgeon may have distinct prognoses. The current study aimed to explore possibility differentially- expressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients tumor node metastasis (TNM) but with different Paracancerous normal tissues taken as controls. specimens histopathologically analyzed. Differentially-expressed miRNAs analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared poor prognosis, those good prognosis exhibited 88 two-fold or more than increased miRNA fragments and 4 half-decreased miRNAs. most noticeably up-regulated included hsa-miR-31, hsa-miR- 196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, hsa-miR-598, whereas significantly- downregulated hsa-let-7e, hsa-miR-130b, hsa-miR-103. TNM prognoses show differentially-expressed