Protein-protein Interaction Network Analyses for Elucidating the Roles of LOXL2-delta72 in Esophageal Squamous Cell Carcinoma

• DOI: 10.7314/apjcp.2014.15.5.2345
• Publication Date: 2014-07-10T07:44:16Z
• Authors: Bing-Li Wu; Hai-Ying Zou; Guo-Qing Lv; Ze-Peng Du; Jian-Yi Wu; Pi-Xian Zhang; Li-Yan Xu; En-Min Li
• Keywords: Lysyl Oxidase

Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase (LOX) family, is copper-dependent enzyme that catalyzes oxidative deamination lysine residues on protein substrates. LOXL2 was found to be overexpressed in esophageal squamous cell carcinoma (ESCC) our previous research. We later identified splicing variant LOXL2-delta72 and we its wild type counterpart ESCC cells following microarray analyses. First, differentially expressed genes (DEGs) compared empty plasmid were applied generate protein-protein interaction (PPI) sub-networks. Comparison these two sub-networks showed hundreds different proteins. To reveal potential specific roles LOXL2- delta72 type, DEGs vs also construct PPI sub-network which annotated by Gene Ontology. The functional annotation map indicated third involved GO terms, such as "cell cycle arrest", "G1/S transition mitotic cycle", "interphase", "cell-matrix adhesion" "cell-substrate adhesion", well significant "immunity" related "innate immune response", "regulation defense response" "Toll signaling pathway". These results provide important clues for experimental identification biological molecular mechanisms LOXL2-delta72. This study provided work flow test with high-throughput data.