Increased Oxidative Stress and RUNX3 Hypermethylation in Patients with Hepatitis B Virus-Associated Hepatocellular Carcinoma (HCC) and Induction of RUNX3 Hypermethylation by Reactive Oxygen Species in HCC Cells

• DOI: 10.7314/apjcp.2015.16.13.5343
• Publication Date: 2015-10-14T02:18:11Z
• Authors: Poonsin Poungpairoj; Patcharawalai Who...; Surasit Suwannasin; Apichaya Khlaiphu...; Pisit Tangkijvanich; Chanchai Boonla
• Keywords: Male; 0301 basic medicine; Hepatitis B virus; Carcinoma, Hepatocellular; Liver Neoplasms; DNA, Neoplasm; DNA Methylation; Middle Aged; Hepatitis B; Prognosis; Polymerase Chain Reaction; 3. Good health; Protein Carbonylation; Oxidative Stress; 03 medical and health sciences; Case-Control Studies; Leukocytes, Mononuclear; Humans; Female; Promoter Regions, Genetic; Reactive Oxygen Species; Follow-Up Studies; Neoplasm Staging

Promoter hypermethylation of the runt-related transcription factor 3 (RUNX3) gene is associated with increased risk hepatocellular carcinoma (HCC). Oxidative stress plays a vital role in both carcinogenesis and progression HCC. However, whether oxidative RUNX3 HCC have cause- and-effect relationship not known. In this study, plasma protein carbonyl total antioxidant capacity (TAC) patients hepatitis B virus (HBV)-associated (n=60) age-matched healthy subjects (n=80) was determined. methylation peripheral blood mononuclear cells (PBMC) measured by methylation-specific PCR. Effect reactive oxygen species (ROS) on induction investigated. Plasma content significantly higher, whereas TAC lower, than controls. Based logistic regression, decreased were independently for PBMC patient group greater group. hydrogen peroxide (H2O2)-treated HepG2 higher untreated control cells. conclusion, increase Thai HBV-associated demonstrated. This increment an development. found to be hypermethylated patients. vitro, experimentally induced H2O2. Our findings suggest that cause promoter