[Application of colloidal gold method and chemiluminescence method for detecting gonadotropins in morning urine to assess pubertal development status in children].

• DOI: 10.7499/j.issn.1008-8830.2409066
• Publication Date: 2025-02-15
• Authors: Xueqi Zhao; Wenli Lü; Wenying Li; Junqi Wang; Zhiya Dong; Yuan Xiao; Xiaofei Zhang; Li Jiang; Xiaoyü Ma
• Keywords: Gold standard (test)

To explore the application of colloidal gold method and chemiluminescence in detecting gonadotropin (Gn) morning urine for assessing pubertal development status children. A total 132 children diagnosed with central precocious puberty (CPP), early fast (EFP), premature thelarche (PT) at Ruijin Hospital Affiliated to Shanghai Jiao Tong University School Medicine from November 2021 December 2022 were included, along 685 healthy who underwent routine health examinations hospital's pediatric care department during same period. All patients a gonadotropin-releasing hormone (GnRH) stimulation test. Both had their urinary Gn levels measured using method, including luteinizing (LH) follicle-stimulating (FSH). The correlation between serum detected by two methods, as well Tanner stages Gn, was analyzed. Urine both showed positive LH baseline values, peak LH/FSH ratios, ratios (P<0.05). In children, gradually increased stage Ⅰ Ⅳ (P<0.05), while FSH lower I than Ⅱ, Ⅲ, IV Urinary compared IV, highest observed Additionally, Ⅲ higher Ⅱ area under receiver operating characteristic curve evaluating II 0.730, 0.699, 0.783, respectively. show good levels. As non-invasive convenient detection can serve useful tool screening onset