[Multi-modal magnetic resonance imaging assessment and mechanism exploration of preterm white matter injury in neonatal rats].

• DOI: 10.7499/j.issn.1008-8830.2411071
• Publication Date: 2025-03-15
• Authors: Xiaotian Gao; Haimo Zhang; X N Zhang; Yijing Wang; Hui-Ning Bi; Miao Yu; Yan Li; Xiaoli Wang

To evaluate preterm white matter injury (PWMI) in neonatal rats using multimodal magnetic resonance imaging (MRI) combined with histological assessments and to explore its underlying mechanisms. Healthy 3-day-old Sprague-Dawley were randomly divided into a sham operation group PWMI (n=12 each group). A model was established through hypoxia-ischemia. Laser speckle used observe changes cerebral oxygen saturation blood flow at different time points post-modeling. Multimodal MRI employed assess the condition of injury, while hematoxylin-eosin staining utilized morphological striatal area on injured side. Immunofluorescence performed detect proliferation differentiation oligodendrocyte precursor cells. At 0, 6, 12, 24, 72 hours post-modeling, relative side significantly lower than those (P<0.05). 24 T2-weighted showed high signals group, apparent diffusion coefficient values Lorenz differential being (P<0.001); additionally, arrangement nerve cells disordered, number EdU+PDGFR-α+ higher that (P<0.001). 28 days fractional anisotropy values, EdU+Olig2+ cells, fluorescence intensity myelin basic protein neurofilament 200 region all can early long-term rat models vivo, providing both pathological evidence for diagnosis treatment neonates. Hypoxia-ischemia inhibits rats, leading PWMI.