[Inhibitory effect of human umbilical cord-derived mesenchymal stem cells on interleukin-17 production in peripheral blood T cells from spondyloarthritis patients].

• DOI: 10.7534/j.issn.1009-2137.2013.02.041
• Publication Date: 2013-04-01
• Authors: Zhifang Huang; Jian Zhu; Shuanghong Lü; Jianglin Zhang; Xian-Da Chen; Du Lixin; Zhigang Yang; Yakun Song; Dong-Ying Wu; Bing Liu; Feng Huang
• Keywords: T-Lymphocytes; Interleukin-17; Spondylarthritis; Leukocytes, Mononuclear; Humans; Mesenchymal Stem Cells; Lymphocyte Count; Umbilical Cord

In this study, the inhibitory effect of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on interleukin-17 (IL-17) production in peripheral blood T from patients with spondyloarthritis (SpA) were investigated, order to explore therapeutic potential hUCMSC SpA. Peripheral mononuclear (PBMNC) isolated SpA (n = 12) and healthy subjects 6). PBMNC cultured vitro or alone. The expression IL-17 CD4(+) γ/δ determined each subject group by flow cytometry. concentrations culture supernatants measured ELISA. results indicated that proportion IL-17-producing 4.5 folds 5 controls [CD3(+)CD4(+)IL-17(+) (3.42 ± 0.82)% vs (0.75 0.25)%, P < 0.01; CD3(+)γδTCR(+)IL-17(+) (0.30 0.10)% (0.06 0.02)%, 0.01]. After co-culture hUCMSC, increased proportions CD3(+)CD4(+)IL-17(+) inhibited significantly (1.81 0.59)% (P 0.01); (0.16 0.06)% response phytohemagglutinin (PHA, 1 µg/ml), secreted more than control [(573.95 171.68) pg/ml (115.53 40.41) 0.01)]. presence produced less amount (443.20 147.94) pg/ml, It is concluded can be which have for