[Identification of a Rare Ael05/B101 Subtype and Selection of Blood Transfusion Strategy].

• DOI: 10.7534/j.issn.1009-2137.2017.05.044
• Publication Date: 2017-10-01
• Authors: Qiang Feng; Jianyu Xiao; Minhui Wu; Yuexiang Zhang; Yong Xia; Xin Mu; Qing Chen
• Keywords: Phenotype; Base Sequence; Blood Grouping and Crossmatching; Genotype; Humans; Exons; Alleles; ABO Blood-Group System

To explore the mechanism of ABO discrepancy in a patient by genotyping and reasonable blood transfusion strategy.Routine serological test was carried out to analyze group. The presence group determinants on red cells were determined adsorption-elution test. Exons 1-7 adjacent introns gene amplified PCR sequenced.The showed forward reverse typing discrepancy. defined as B, however, indicated that AB subtype. Absorption-elution confirmed weak A antigens patient's cells. sequencing T>C exchange at position exon 7 which resulted isoleucine threonine substitution codon 256. genotype ABO*Ael05/B101.The 767 α-1,3-N-acetyl galactose is molecular leading decrease expression antigen Ael05