The kinetochore prevents centromere-proximal crossover recombination during meiosis

• DOI: 10.7554/elife.10850
• Publication Date: 2015-12-14T12:35:27Z
• Authors: Nadine Vincenten; Lisa-Marie Kuhl; Isabel Lam; Ashwini Oke; Alastair RW Kerr; Andreas Hochwagen; Jennifer Fung; Scott Keeney; Gerben Vader; Adèle L Marston
• Keywords: Nondisjunction

During meiosis, crossover recombination is essential to link homologous chromosomes and drive faithful chromosome segregation. Crossover non-random across the genome, centromere-proximal crossovers are associated with an increased risk of aneuploidy, including Trisomy 21 in humans. Here, we identify conserved Ctf19/CCAN kinetochore sub-complex as a major factor that minimizes potentially deleterious budding yeast. We uncover multi-layered suppression pericentromeric by Ctf19 complex, operating distinct chromosomal distances. The complex prevents meiotic DNA break formation, initiating event recombination, proximal centromere. independently drives enrichment cohesin throughout broader pericentromere suppress crossovers, but not breaks. This non-canonical role defining domain refractory adds new layer functionality which incidence segregation errors generate aneuploid gametes.