A filter at the entrance of the Golgi that selects vesicles according to size and bulk lipid composition

• DOI: 10.7554/elife.16988
• Publication Date: 2016-07-28T14:48:06Z
• Authors: Maud Magdeleine; Romain Gautier; Pierre Gounon; Hélène Barelli; Stefano Vanni; Bruno Antonny
• Keywords: Organelle

When small phosphatidylcholine liposomes are added to perforated cells, they bind preferentially the Golgi suggesting an exceptional avidity of this organelle for curved membranes without stereospecific interactions. We show that cis golgin GMAP-210 accounts property. First, liposome tethering properties resembles amphipathic lipid-packing sensor (ALPS) motif GMAP-210: both preferred (radius < 40 nm) made monounsaturated but not saturated lipids. Second, reducing levels or redirecting its ALPS mitochondria decreased capture by Golgi. Extensive mutagenesis analysis suggests tethers authentic transport vesicles via same mechanism whereby senses defects at vesicle surface through regularly spaced hydrophobic residues. conclude uses as a filter select according their size and bulk lipid composition.