Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis

• DOI: 10.7554/elife.24071
• Publication Date: 2017-03-29T16:00:39Z
• Authors: Yu A An; Kai Sun; Nolwenn Joffin; Fang Zhang; Yingfeng Deng; Olivier Donzé; Christine M Kusmi...; Philipp E Scherer
• Keywords: Angiopoietin; Homeostasis; FGF21; Angiopoietin receptor

Despite many angiogenic factors playing crucial roles in metabolic homeostasis, effects of angiopoietin-2 (ANG-2) adipose tissue (AT) remain unclear. Utilizing a doxycycline-inducible AT-specific ANG-2 overexpression mouse model, we assessed the AT expansion upon high-fat diet (HFD) challenge. is significantly induced, with subcutaneous white (sWAT) displaying highest expression. overexpressing mice show increased sWAT vascularization and are resistant to HFD-induced obesity. In addition, improved glucose lipid metabolism observed. Mechanistically, displays healthier pattern anti-inflammatory macrophage infiltration. Conversely, neutralization HFD-challenged wild-type shows reduced sWAT, associated impaired tolerance clearance. Blocking causes significant pro-inflammatory pro-fibrotic changes, hallmarks an unhealthy expansion. contrast other pro-angiogenic factors, such as vascular endothelial growth factor-A (VEGF-A), this achieved without any enhanced beiging AT.