Robo2 regulates synaptic oxytocin content by affecting actin dynamics

• DOI: 10.7554/elife.45650
• Publication Date: 2019-06-10T12:00:35Z
• Authors: Savani Anbalagan; Janna Blechman; Michael Gliksberg; Ludmila Gordon; Ron Rotkopf; Tali Dadosh; Eyal Shimoni; Gil Levkowitz
• Keywords: Cofilin

The regulation of neuropeptide level at the site release is essential for proper neurophysiological functions. We focused on a prominent neuropeptide, oxytocin (OXT) in zebrafish as an vivo model to visualize and quantify OXT content resolution single synapse. found that OXT-loaded synapses were enriched with polymerized actin. Perturbation actin filaments by either cytochalasin-D or conditional Cofilin expression resulted decreased synaptic levels. Genetic loss robo2 slit3 displayed mutants reduced mobility probe Lifeact-EGFP synapses. Using novel transgenic reporter allowing real-time monitoring vesicles, we show display slower rate vesicles accumulation. OXT-specific dominant-negative Cdc42, which key regulator dynamics downstream effector Robo2, led dose-dependent increase WT, dampened effect mutants. Our results link Slit3-Robo2-Cdc42, controls local dynamics, maintenance