Age-dependent deterioration of nuclear pore assembly in mitotic cells decreases transport dynamics

• DOI: 10.7554/elife.48186
• Publication Date: 2019-06-03T12:00:21Z
• Authors: Irina L Rempel; Matthew M Crane; David J Thaller; Ankur Mishra; Daniel PM Jansen; Georges Janssens; Petra Popken; Arman Akşit; Matt Kaeberlein; Erik van der Giessen; Anton Steen; Patrick R Onck; C Patrick Lusk; Liesbeth M Veenhoff
• Keywords: Nuclear pore; Interphase; Compartmentalization (fire protection)

Nuclear transport is facilitated by the Pore Complex (NPC) and essential for life in eukaryotes. The NPC a long-lived exceptionally large structure. We asked whether quality control compromised aging mitotic cells. Our images of single yeast cells during aging, show that abundance several components assembly factors decreases. Additionally, single-cell histories reveal better maintain those are longer lived. presence herniations at nuclear envelope aged suggests misassembled NPCs accumulated Aged decreased dynamics transcription factor shuttling increased compartmentalization. These functional changes likely caused NPCs, as we find two mutants similar phenotypes conclude interphase major challenge