A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells

• DOI: 10.7554/elife.48221
• Publication Date: 2019-09-25T12:00:14Z
• Authors: Enas Abu-Shah; Philippos Demetriou; Štefan Bálint; Viveka Mayya; Mikhail A Kutuzov; Omer Dushek; Michael L Dustin
• Keywords: CD4-Positive T-Lymphocytes; 0301 basic medicine; QH301-705.5; Science; Antigen-Presenting Cells; Cell Communication; antigen presenting cells; CD8-Positive T-Lymphocytes; Lymphocyte Activation; 03 medical and health sciences; Humans; Biology (General); Antigens; Human Biology and Medicine; Cells, Cultured; Antigen Presentation; T-cells; Q; R; Dendritic Cells; TCR engineering; 3. Good health; Immune System; Medicine; Cytokines; immunotherapy; 3D model system

Research in the field of human immunology is restricted by lack a system that reconstitutes in-situ activation dynamics quiescent antigen-specific T-cells interacting with dendritic cells. Here we report tissue-like recapitulates engineered primary immune cell. Our approach facilitates real-time single-cell manipulations, tracking interactions and functional responses complemented population-based measurements cytokines, status proliferation. As proof concept, recapitulate immunological phenomenon such as CD4 T-cells' help to CD8 through enhanced maturation DCs effect PD-1 checkpoint blockades. In addition, characterise unique T-cell/DC function antigen affinity.