Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree

• DOI: 10.7554/elife.67776
• Publication Date: 2021-05-18T14:00:35Z
• Authors: Audrey M Hendley; Arjun A Rao; Laura Leonhardt; Sudipta Ashe; Jennifer A Smith; Simone Giacometti; Xianlu L Peng; Honglin Jiang; David I Berrios; Mathias Pawlak; Lucia Y Li; Jonghyun Lee; Eric A Collisson; Mark S Anderson; Gabriela K Fragia...; Jen Jen Yeh; Chun Jimmie Ye; Grace E Kim; Valerie M Weaver; Matthias Hebrok
• Keywords: Cholangiocyte

To study disease development, an inventory of organ's cell types and understanding physiologic function is paramount. Here, we performed single-cell RNA-sequencing to examine heterogeneity murine pancreatic duct cells, pancreatobiliary intrapancreatic bile cells. We describe epithelial-mesenchymal transitory axis in our three subpopulations identify osteopontin as a regulator this fate decision well human dedifferentiation. Our results further functional within by elucidating role for geminin accumulation DNA damage the setting chronic pancreatitis. findings implicate diverse roles cells maintenance identity progression establish comprehensive road map cell, homeostasis.