CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1

• DOI: 10.7554/elife.69602
• Publication Date: 2022-01-14T12:15:14Z
• Authors: Andreas Kolbeck; Peter Marhavý; Damien De Bellis; Baohai Li; Takehiro Kamiya; Toru Fujiwara; Lothar Kalmbach; Niko Geldner
• Keywords: Endodermis; Lipid microdomain; Stele; Ectopic expression; Plasmodesma

Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed plants. Here, show that correct establishment Casparian Strip (CS) network relies on local neighbor communication. We positioning membrane domains (CSDs) is tightly coordinated between neighbors wild-type restriction domain formation involves putative extracellular protease LOTR1. Impaired lotr1 leads to fully functional CSDs at ectopic positions, forming ‘half strips’. LOTR1 action endodermis its expression stele. endodermal cannot complement, while cortex causes a dominant-negative phenotype. Our findings establish as crucial player CSD acting directional, non-cell-autonomous manner restrict coordinate CS positioning.