Visualizing synaptic dopamine efflux with a 2D composite nanofilm

• DOI: 10.7554/elife.78773
• Publication Date: 2022-07-04T12:01:04Z
• Authors: Chandima Bulumulla; Andrew T Krasley; Ben Cristofori-Ar...; William C Valinsky; Deepika Walpita; David Ackerman; David E Clapham; Abraham G Beyene
• Keywords: Neurochemical; Active zone

Chemical neurotransmission constitutes one of the fundamental modalities communication between neurons. Monitoring release these chemicals has traditionally been difficult to carry out at spatial and temporal scales relevant neuron function. To understand chemical more fully, we need improve resolutions measurements for neurotransmitter release. address this, engineered a chemi-sensitive, two-dimensional composite nanofilm that facilitates visualization diffusion neurochemical dopamine with synaptic resolution, quantal sensitivity, simultaneously from hundreds sites. Using this technology, were able monitor spatiotemporal dynamics in dendritic processes, poorly understood phenomenon. We found is broadcast subset processes as hotspots have mean spread ≈ 3.2 µm (full width half maximum [FWHM]) are observed frequency hotspot per 7.5 length. Major dendrites neurons fine well arbors no apparent varicose morphology participated Remarkably, co-localized Bassoon, suggesting Bassoon may contribute organizing active zones dendrites, similar its role axon terminals.