Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration

• DOI: 10.7554/elife.80122
• Publication Date: 2023-02-01T11:03:43Z
• Authors: Eunjin Cho; Xiangguo Che; Mary Jasmin Ang; Seongmin Cheon; Jinkyung Lee; Kwang Soo Kim; Chang Hoon Lee; Sang-Yeop Lee; Hee-Young Yang; Changjong Moon; Chungoo Park; Je-Yong Choi; Tae-Hoon Lee
• Keywords: Peroxiredoxin

Peroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function bone remains largely unknown. Here, we show that Prdx5 osteoclast and osteoblast differentiation, resulting osteoporotic phenotypes knockout (Prdx5Ko) male mice. To investigate of bone, osteoblasts were analyzed through immunoprecipitation (IP) liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) methods, while osteoclasts RNA-sequencing. Heterogeneous nuclear ribonucleoprotein K (hnRNPK) was identified as a potential binding partner during differentiation vitro. acts negative regulator hnRNPK-mediated osteocalcin (Bglap) expression. In addition, transcriptomic analysis revealed vitro differentiated from marrow-derived macrophages Prdx5Ko mice showed enhanced expression several osteoclast-related genes. These findings indicate might contribute to maintenance homeostasis by regulating differentiation. This study proposes new remodeling may be used developing therapeutic strategies for diseases.