Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation

• DOI: 10.7554/elife.91684.3
• Publication Date: 2024-08-13T14:59:01Z
• Authors: Xinyu Chen; Huasong Zou; Tao Zhuo; Wei Rou; Wei Wu; Xiaojing Fan
• Keywords: Xanthomonas citri; Citrus canker; Xanthomonas; Transcription; Canker

Xanthomonas citri subsp. ( Xcc ), the causal agent of citrus canker, elicits canker symptoms in plants because transcriptional activator-like (TAL) effector PthA4, which activates expression susceptibility gene CsLOB1 . This study reports regulation putative carbohydrate-binding protein Cs9g12620 by PthA4-mediated induction during infection. We found that transcription was induced infection with a PthA4-dependent manner. Even though it specifically bound to TAL effector-binding element promoter, PthA4 exerted suppressive effect on promoter activity. In contrast, activate its expression. The silencing significantly reduced level , demonstrated directly regulated CsLOB1. Intriguingly, PhtA4 interacted and feedback control suppressed Transient overexpression revealed required for optimal development symptoms. These results support hypothesis is dynamically directed formation through