Clustered synapses develop in distinct dendritic domains in visual cortex before eye opening

• DOI: 10.7554/elife.93498.1
• Publication Date: 2023-12-28T20:25:22Z
• Authors: Alexandra H. Leig...; Juliette E. Cheyne; Christian Lohmann
• Keywords: Patch-Clamp Techniques; QH301-705.5; Science; spines; Inbred C57BL; neuroscience; Mice; Animals; two-photon microscopy; Biology (General); Dendrites/physiology; mouse; Visual Cortex; Q; patch-clamp methodology; R; Visual Cortex/physiology; Dendrites; in utero electroporation; Mice, Inbred C57BL; calcium imaging; in vivo; Synapses; Medicine; Synapses/physiology; Neuroscience

Synaptic inputs to cortical neurons are highly structured in adult sensory systems, such that neighboring synapses along dendrites activated by similar stimuli. This organization of synaptic inputs, called clustering, is required for high-fidelity signal processing, and clustered can already be observed before eye opening. However, how emerge during development unknown. Here, we employed concurrent vivo whole-cell patch clamp dendritic calcium imaging map spontaneous layer 2/3 the mouse primary visual cortex second postnatal week until We find number functional frequency transmission events increase several fold this developmental period. At beginning week, assemble specifically confined segments, whereas other segments devoid synapses. By end just eye-opening, almost entirely covered domains co-active Finally, co-activity with their neighbor correlates stabilization potentiation. Thus, form distinct presumably equip computational modules high-capacity processing when eyes open.