Maintenance of cell wall remodeling and vesicle production are connected in Mycobacterium tuberculosis

• DOI: 10.7554/elife.94982.3
• Publication Date: 2025-01-06T12:25:29Z
• Authors: Vivian Salgueiro; Jorge Bertol; Claude Gutierrez; Jose L Serrano-Me...; Noelia Ferrer-Luzon; Lucía Vázquez-Ini...; Ainhoa Palacios; Laia Pasquina-Lem...; Akbar Espaillat; Laura Lerma; Brian Weinrick; Jose L Lavin; Felix Elortza; Mikel Azkalgorta; Alicia Prieto; Pilar Buendía-Nac...; José L Luque-García; Olivier Neyrolles; Felipe Cava; Jamie K Hobbs; Joaquín Sanz; Rafael Prados-Ros...
• Keywords: Extracellular vesicles; Cell envelope; Organelle biogenesis

Pathogenic and nonpathogenic mycobacteria secrete extracellular vesicles (EVs) under various conditions. EVs produced by Mycobacterium tuberculosis ( Mtb ) have raised significant interest for their potential in cell communication, nutrient acquisition, immune evasion. However, the relevance of vesicle secretion during infection remains unknown due to limited understanding mycobacterial biogenesis. We previously shown that a transposon mutant LCP-related gene virR mut manifested strong attenuated phenotype experimental macrophage murine infections, concomitant enhanced release. In this study, we aimed understand role VirR production process . employ genetic, transcriptional, proteomics, ultrastructural biochemical methods investigate underlying processes explaining vesiculogenesis phenomenon observed Our results establish is critical sustain proper permeability via regulation envelope remodeling possibly through interaction with similar proteins, which control link between peptidoglycan arabinogalactan. These findings advance our biogenesis suggest these set proteins could be attractive targets therapeutic intervention.