The Geometric Basis of Epithelial Convergent Extension

• DOI: 10.7554/elife.95521.2
• Publication Date: 2024-07-29T10:26:48Z
• Authors: Fridtjof Brauns; Nikolas H Claussen; Matthew F Lefebvre; Eric F Wieschaus; Boris I Shraiman
• Keywords: active cell intercalation; 0303 health sciences; QH301-705.5; Science; Q; R; self-organization; Article; 03 medical and health sciences; convergent extension; tissue mechanics; passive cell intercalation; Medicine; Biology (General); Developmental Biology

Shape changes of epithelia during animal development, such as convergent extension, are achieved through concerted mechanical activity of individual cells. While much is known about the corresponding large scale tissue flow and its genetic drivers, fundamental questions regarding local control of contractile activity on cellular scale and its embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry to local tension in recently obtained timelapse imaging data of gastrulating Drosophila embryos. This analysis provides a systematic decomposition of cell shape changes and T1-rearrangements into internally driven, active, and externally driven, passive, contributions. Our analysis provides evidence that germ band extension is driven by active T1 processes that self-organize through positive feedback acting on tensions. More generally, our findings suggest that epithelial convergent extension results from controlled transformation of internal force balance geometry which combines the effects of bottom-up local self-organization with the top-down, embryo-scale regulation by gene expression.