Silicone Wire Embolization-induced Acute Retinal Artery Ischemia and Reperfusion Model in Mouse: Gene Expression Provide Insight into Pathological Processes

• DOI: 10.7554/elife.98949.1
• Publication Date: 2024-06-13T17:29:39Z
• Authors: Yuedan Wang; Ying Li; Jiaqing Feng; Chuansen Wang; Yuwei Wan; Bingyang Lv; Yinming Li; Hao Xie; Ting Chen; Faxi Wang; Ziyue Li; Anhuai Yang; Xuan Xiao
• Keywords: Ganglion cell layer; Inner nuclear layer

Acute retinal ischemia and ischemia-reperfusion injury are primary causes of neural cell death vision loss in artery occlusion (RAO). The absence an accurate mouse model simulating the ischemic process has hampered progress developing neuroprotective agents for RAO. A unilateral pterygopalatine ophthalmic (UPOAO) was developed by employing silicone wire embolization combined with carotid ligation. survival ganglion cells visual function were evaluated to determine duration. Immunofluorescence staining, optical coherence tomography, hematoxylin eosin staining utilized assess changes major classes structure degeneration at two reperfusion durations. Transcriptomics employed investigate alterations pathological UPOAO following reperfusion, highlighting transcriptomic differences between other models. successfully replicated acute interruption blood supply seen 60-minute confirmed lead impairment. Notable thinning inner layer retina, especially layer, evident post-UPOAO. Temporal transcriptome analysis revealed various pathophysiological processes related immune migration, oxidative stress, inflammation during non-reperfusion periods. resident microglia within retina peripheral leukocytes which access pronounced increased on Comparison differentially expressed genes high intraocular pressure models identified specific enrichments lipid steroid metabolism-related model. emerges as a novel tool screening pathogenic genes, promoting further therapeutic research