Effect of Canine Adipose Mesenchymal Stem Cell Secretome on a Model of Second-Intention Wound Healing in the Red-Eared Slider Turtle (Trachemys scripta)

• DOI: 10.7589/jwd-d-20-00100
• Publication Date: 2022-04-25T12:42:32Z
• Authors: Sayda Pérez-Delgado; Pedro J. Ginel; Rafael Guerra; Elena Mozos; Cristina Alcoholado; María del Carmen ...; José Becerra; Antonio J. Villatoro
• Keywords: Male; 0403 veterinary science; Wound Healing; Dogs; Animals; Female; Mesenchymal Stem Cells; Intention; 04 agricultural and veterinary sciences; Secretome; Turtles

Mesenchymal stem cell (MSC) secretome refers to a variety of bioactive compounds that represents the more important pathway by which MSCs participate in tissue regeneration. Many these have shown variable functional activity even across nonmammalian vertebrate species, although turtles not yet been described. Canine adipose MSC has successfully used experimentally skin healing. Our aim was conduct blinded controlled study evaluate effect canine (cS-MSC) as an alternative for healing soft skin, second intention wounds red-eared slider (Trachemys scripta). Under general anesthesia, one circular, 6-mm full thickness wound made each rear leg 14 females. After randomization, cS-MSC injected subcutaneously around at days 1, 7, and 14, whereas other acted control. Biopsies from three animals' were obtained 21, 28, 42, 63. Differences mean retraction 21 (n=14) 28 (n=11) statistically nonsignificant. The clinical histopathologic scores performed blind two different investigators similar treated control wounds. In conclusion, we could detect significant on Trachemys scripta.