Expansión clónica y caracterización genómica del proceso de integración del virus linfotrópico humano tipo I en la leucemia/linfoma de células T en adultos

• DOI: 10.7705/biomedica.v29i2.24
• Publication Date: 2014-07-29T20:03:21Z
• Authors: Mercedes Salcedo-...; Jesús Cabrera; Yesid Cuesta-Astroz; Edwin Carrascal; Yoshito Eizuru; Martha C. Domínguez; Adalberto Sánchez; Felipe García-Val...
• Keywords: Adult; DNA Replication; Male; Adolescent; polymerase chain reaction; RC955-962; genoma humano; Genome, Viral; Virus integration; leukaemia-lymphoma; computational biology; Virus integration, human T-lymphotropic virus 1; Arctic medicine. Tropical medicine; Humans; Leukemia-Lymphoma, Adult T-Cell; Child; integración viral; adult T-cell; Aged; biología computacional; human T-lymphotropic virus 1; Aged, 80 and over; Base Composition; Human T-lymphotropic virus 1; Genes, pX; virus linfotrópico de células T humanas tipo 1; R; DNA, Neoplasm; Cell Transformation, Viral; reacción en cadena de la polimerasa; leukaemia-lymphoma, adult T-cell; Clone Cells; Genes, cdc; Child, Preschool; DNA, Viral; Medicine; genome, human; Female; leucemia/linfoma de células T en adultos

Introduction. Although the integration of human T-cell lymphotropic virus type I into T-cells is not a random process, mechanistic details are understood.Objectives. The characteristics flanking host chromatin were evaluated at sites in adult leukaemia/lymphoma (ATLL) patients infected with virus.Materials and methods. From seven leukemic Colombian positive for (HTLV-I), lymphocyte DNA samples extracted amplified by inverse polymerase chain reaction (IPCR). Clonal expansion genome nucleotide composition an extension 50 bp was determined. To establish provirus, 61 IPCR sequences from Japanese ATLL patients, analyzed silico to obtain insights about genomic structure, functions nature associated chromatin.Results. clonal cell clones predominantly oligoclonal. sequences, 155 alignments homology higher than 95% (e-value <0.05) screened. Seventy-five percent those corresponded non coding elements that include repetitive non-repetitive DNA. Fifty proviral integrations chromosomes A B groups. Viral tended favor exons genes replicated early, controlled cycle, or involved signal transduction.Conclusions. results indicated HTLV-I preferentially directed towards environments high C:G content, toward replicate regulate cycle transduction.