PPBP gene as a biomarker for coronary heart disease risk in postmenopausal Thai women

• DOI: 10.7717/peerj.13615
• Publication Date: 2022-06-17T08:51:33Z
• Authors: Chayasin Mansanguan; Yaowapa Maneerat
• Keywords: Male; QH301-705.5; Epidemiology; Peroxisome Proliferator-Activated Receptors; Framingham Risk Score; Coronary Disease; Hyperlipidemias; Cholesterol-lowering; 03 medical and health sciences; 0302 clinical medicine; Biochemistry, Genetics and Molecular Biology; Health Sciences; Genetics; Humans; Disease; RNA, Messenger; Biology (General); Molecular Biology; Internal medicine; Biology; PPBP; R; Southeast Asian People; Life Sciences; Estrogens; Biomarker; Atherosclerosis; Postmenopausal women; 3. Good health; Postmenopause; Inflammation and Obesity-Related Metabolic Disorders; Coronary heart disease; Cross-Sectional Studies; Cholesterol-lowering Treatment; FOS: Biological sciences; Leukocytes, Mononuclear; Medicine; Female; Surgery; Biomarkers

Background Estrogen is an important ovarian hormone with anti-atherogenic and cardioprotective effects. Postmenopausal women have lower estrogen levels, associated significantly higher risks of coronary heart disease (CHD) CHD-related death. Effective biomarkers for the diagnosis, prediction, treatment CHD are needed to address this problem thus reduce mortality due in postmenopausal women. We recently reported that PPBP DEFA1/DEFA3 genes may be feasible synergistic risk Thai men hyperlipidemia. The gene encodes pro-platelet basic protein (PPBP) from activated platelets, human neutrophil peptides (HNP) 1–3, mainly produced by neutrophils. Both platelets neutrophils involved chronic inflammation during development atherogenesis CHD. This study investigated potential roles their proteins as Methods cross-sectional enrolled 90 women, including 12 healthy controls (N), 18 patients hyperlipidemia (H), 21 diagnosed remaining 39 were receiving cholesterol-lowering drugs (HD) excluded study. All underwent bypass grafting or angioplasty. mRNA expression levels peripheral blood mononuclear cells isolated heparinized determined quantitative reverse-transcription polymerase chain reaction. Levels HNP-1–3 corresponding plasma samples assessed enzyme-linked immunosorbent assay. Differences parameters compared among groups correlations between clinical manifestations analyzed. Results increased group N H groups. In contrast, did not differ None any analyzed Conclusion results indicate PPBP, but , HNP-1–3, assessing