Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells

• DOI: 10.7717/peerj.1483
• Publication Date: 2015-12-08T17:25:49Z
• Authors: Harpa Karadottir; Nikhil Nitin Kulk...; Thorarinn Gudjonsson; Sigurbergur Karason; Gudmundur Hrafn G...
• Keywords: Cathelicidin; Inflammatory response; Beta defensin

Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects MV innate immune defense system human lung. In this study, we demonstrate that cyclic stretch bronchial epithelial cell lines VA10 BCi NS 1.1 leads down-regulation cathelicidin antimicrobial peptide (CAMP) gene expression. We show treatment cells with vitamin D3 and/or 4-phenyl butyric acid counteracted mediated CAMP mRNA protein expression (LL-37). Further, observed an increase pro-inflammatory responses line subjected stretch. The genes encoding cytokines IL-8 IL-1β was increased after stretching, where as decrease chemokines IP-10 RANTES observed. Cyclic enhanced oxidative stress cells. toll-like receptor (TLR) 3, TLR5 TLR8 reduced, while TLR2 conclusion, our vitro results indicate may differentially modulate immunity by responses.