An alternative peptone preparation using Hermetia illucens (Black soldier fly) hydrolysis: process optimization and performance evaluation

• DOI: 10.7717/peerj.16995
• Publication Date: 2024-02-26T08:15:36Z
• Authors: Gaoqiang Liu; Ming Foong Tiang; Shixia Ma; Zeyan Wei; Xiaolin Liang; Mohd Shaiful Sajab; Peer Mohamed Abdul; Xueyan Zhou; Zhongren Ma; Gongtao Ding
• Keywords: Hermetia illucens; Ingredient; Enzymatic Hydrolysis

Background Hermetia illucens (HI), commonly known as the black soldier fly, has been recognized for its prowess in resource utilization and environmental protection because of ability to transform organic waste into animal feed livestock, poultry, aquaculture. However, potential fly’s high protein content more than cheap feedstock is still largely unexplored. Methods This study innovatively explores H. larvae (HIL) a peptone substitute microbial culture media. Four commercial proteases (alkaline protease, trypsin, trypsase, papain) were explored hydrolyze defatted HIL, experimental conditions optimized via response surface methodology design. The hydrolysate HIL was subsequently vacuum freeze-dried deployed growth medium three bacterial strains ( Staphylococcus aureus , Bacillus subtilis Escherichia coli ) determine kinetics between peptone. Results optimal 1.70% w/w complex enzyme protease: trypsin at 1:1 ratio) pH 7.0 54 °C duration 4 h. Under these conditions, hydrolysis yielded 19.25% ±0.49%. A kinetic analysis showed no significant difference parameters μ max X λ peptone, demonstrating that could serve an effective, low-cost alternative introduces innovative approach utilization, broadening application beyond current use feed.