Overexpression of OsFTL10 induces early flowering and improves drought tolerance in Oryza sativa L.

• DOI: 10.7717/peerj.6422
• Publication Date: 2019-02-12T08:17:01Z
• Authors: Maichun Fang; Zejiao Zhou; Xusheng Zhou; Huiyong Yang; Meiru Li; Hongqing Li
• Keywords: Bimolecular fluorescence complementation; Genetically modified rice; Drought stress; Drought Tolerance

Flowering time control is critically important for the reproductive accomplishment of higher plants as floral transition can be affected by both environmental and endogenous signals. Locus T-like (FTL) genes are major genetic determinants flowering in plants. In rice, 13 OsFTL have been annotated genome amongst them, Hd3a (OsFTL2) RFT1 (OsFTL3) studied extensively their functions confirmed central florigens that rice under short day long environment, respectively. this report, a gene, OsFTL10, was characterized, its function on abiotic stress investigated. The expression level OsFTL10 high young seedlings shown to induced GA3 drought stress. Overexpression resulted earlier up 2 weeks, through up-regulation downstream gene OsMADS15. also regulated Ehd1 OsMADS51 feedback mechanism. protein detected nucleus cytoplasm. Furthermore, yeast two hybrid (Y2H) bimolecular fluorescence complementation (BiFC) results show could interact with multiple 14-3-3s, suggesting might similar way promoting forming FAC complex 14-3-3, OsFD1. Further experiments revealed constitutive improved tolerance transgenic stimulating responsive genes. These suggest FTL promotion responses