The prevalence ofPseudomonas aeruginosaand multidrug resistantPseudomonas aeruginosain healthy captive ophidian

• DOI: 10.7717/peerj.6706
• Publication Date: 2019-04-12T08:23:46Z
• Authors: Andrea Sala; Francesco Di Ianni; Igor Pelizzone; Mara Bertocchi; Davide Santospirito; Francesco Rogato; Sara Flisi; Costanza Spadini; Tiziano Iemmi; Emanuele Moggia; Enrico Parmigiani; Sandro Cavirani; Simone Taddei; Clotilde S. Cabassi
• Keywords: QH301-705.5; Farming condition; Antibiotic; R; 610; Snakes; Ophidians; 04 agricultural and veterinary sciences; Ophidian; Microbiology; 3. Good health; 0403 veterinary science; Multidrug resistant; Pseudomonas aeruginosa; Captive; Prevalence; Medicine; Antimicrobial; Biology (General)

Snakes are globally considered as pet animals, and millions of ophidians bred in captivity. Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can act an opportunistic pathogen man animals frequently present the oral cloacal microbiota healthy ophidians. It cause severe clinical diseases often shows antibiotic resistance. The aim this study was to evaluate prevalence resistance profiles P. isolated from large population sample captive statistical associations with farming conditions.A total 419 swabs were collected snakes belonging Boidae (n = 45), Colubridae 48) Pythonidae 326) families inoculated onto complete culture media. Food, water bedding samples also analyzed. antimicrobial susceptibility isolates evaluated through Kirby-Bauer agar diffusion test. Statistical analyses performed chi-square test.The 59.9%, 35.5% these strains multidrug resistant (MDR). MDR significantly higher adult than young samples, widespread Cephalosporins, Polymyxins Sulfonamides observed. Statistically significant differences observed depending on farm size snake family. Feeding thawed prey associated prevalence. Moreover, fed home-raised had commercially available feed. Less frequent terrarium cleaning On other hand, reproductive status not or All food, negative for presence.The overall found lower by authors, but high proportion MDR. This highlighted presence constitutive (such age taxonomic family) managerial (farm size, cycle frequency food type) factors and/or Good breeding management proper treatment infections could help reduce gut consequently risk public health.