Determination of the evolutionary pressure onCamellia oleiferaon Hainan Island using the complete chloroplast genome sequence

• DOI: 10.7717/peerj.7210
• Publication Date: 2019-06-26T07:13:51Z
• Authors: Wan Zhang; Yunlin Zhao; Guiyan Yang; Jiao Peng; Shuwen Chen; Zhenggang Xu
• Keywords: Camellia oleifera; Germ plasm

Camellia oleifera is one of the four largest woody edible oil plants in world with high ecological and medicinal values. Due to frequent interspecific hybridization, it was difficult study its genetics evolutionary history. This used C. that collected on Hainan Island conduct our research. The unique island environment makes quality tea higher than other species grown mainland. Moreover, a long-term geographic isolation might affect gene structure. In order better understand molecular biology this species, protect excellent germplasm resources, promote population phylogenetic studies plants, high-throughput sequencing technology obtain chloroplast genome sequence oleifera. results showed whole 156,995 bp length, typical quadripartite structure large single copy (LSC) region 86,648 bp, small (SSC) 18,297 pair inverted repeats (IRs) 26,025 bp. encoded total 141 genes (115 different genes), including 88 protein-coding genes, 45 tRNA eight rRNA genes. Among these nine contained intron, two introns, overlapping were also detected. GC content oleifera's 37.29%. characteristics compared mainland those closely related Theaceae species; found contractions expansions IR/LSC IR/SSC regions affected length genome. sequences highly similar. A comparative analysis indicated conserved evolution. 51 simple repeat (SSR) loci detected oleifera, all did not have pentanucleotide repeats, which could be as good marker studies. We seven long base composition biased toward A/T, consistent codon bias. It had similar relationship crapnelliana, through use codons analysis. can provide an effective genomic resource for history family.