Age-related Effects of Heroin on Gene Expression in the Hippocampus and Striatum of Cynomolgus Monkeys

• DOI: 10.9758/cpn.2020.18.1.93
• Publication Date: 2020-01-20T21:50:23Z
• Authors: Mi Ran Choi; Yeung-Bae Jin; Sol Hee Bang; Chang-Nim Im; Youngjeon Lee; Han-Na Kim; Kyu-Tae Chang; Sang-Rae Lee; Dai-Jin Kim
• Keywords: 0301 basic medicine; 03 medical and health sciences; Original Article

The aim of this study was to investigate differentially expressed genes and their functions in the hippocampus striatum after heroin administration cynomolgus macaques different ages.Cynomolgus monkeys were divided by age as follows: 1 year (A1, n = 2); 3 4 years (A2, 6 8 (A3, older than 11 (A4, 2). After injected intramuscularly into (0.6 mg/kg), we performed large-scale transcriptome profiling (H) (S) using RNA sequencing technology. Some validated with real-time quantitative PCR.In hippocampus, gene expression A1H similar that A4H, while A2H A3H. Genes associated mitogen-activated protein kinase signaling pathway (STMN1, FGF14, MAPT) γ-aminobutyric acid-ergic synapses (GABBR2 GAD1) among control heroin-treated animals. Differential between A1S A4S least significant, differential A3S A2S most significant. neurotrophin (NTRK1 NGFR), autophagy (ATG5), dopaminergic (AKT1) animals.These results suggest even a single exposure can cause nonhuman primates at ages.