A5049223044- Toxic Organic Pollutants Impact
- Heavy metals in environment
- Nanoparticles: synthesis and applications
- Effects and risks of endocrine disrupting chemicals
- Environmental Toxicology and Ecotoxicology
- Neuroscience and Neuropharmacology Research
- Air Quality and Health Impacts
- Mercury impact and mitigation studies
- Microplastics and Plastic Pollution
- Heavy Metal Exposure and Toxicity
- Pesticide Exposure and Toxicity
- Carcinogens and Genotoxicity Assessment
- Arsenic contamination and mitigation
- 3D Printing in Biomedical Research
- Per- and polyfluoroalkyl substances research
- Molecular Sensors and Ion Detection
- Insect and Pesticide Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Recycling and Waste Management Techniques
- Isotope Analysis in Ecology
- Advanced biosensing and bioanalysis techniques
- Inhalation and Respiratory Drug Delivery
- Cerebrospinal fluid and hydrocephalus
- Computational Drug Discovery Methods
- Biotechnology and Related Fields
NILU
2010-2024
Norwegian Institute of Public Health
2021-2024
Norwegian Defence Research Establishment
2010-2022
University of Oslo
2009
(1) In compliance with the 3Rs policy to reduce, refine and replace animal experiments, development of advanced in vitro models is needed for nanotoxicity assessment. Cells cultivated 3D resemble organ structures better than 2D cultures. This study aims compare cytotoxic genotoxic responses induced by titanium dioxide (TiO2), silver (Ag) zinc oxide (ZnO) nanoparticles (NPs) monolayer spheroid cultures HepG2 human liver cells. (2) NPs were characterized electron microscopy, dynamic light...
Tetrabromobisphenol-A (TBBPA) is one of the worlds most widely used brominated flame retardant. The present study reports effects TBBPA on primary cultures cerebellar granule cells (CGC). Using trypan blue exclusion assay, we show that induces death CGC at low micro molar concentrations. Cell was reduced by NMDA receptor antagonist MK-801 (3μM), antioxidant vitamin E (50μM), and in calcium-free buffer. We further demonstrate TBBPA's toxicity accompanied apoptosis-like nuclear shrinkage,...
In accordance with the 3 Rs to reduce in vivo testing, more advanced vitro models, moving from 2D monolayer 3D cultures, should be developed for prediction of human toxicity industrial chemicals and environmental pollutants. this study we compared cytotoxic genotoxic responses induced by spheroidal cultures liver cancer cell line HepG2. HepG2 spheroids were prepared hanging drop technology. Both exposed different (colchicine, chlorpromazine hydrochloride or methyl methanesulfonate) geno-...
BACKGROUND. Methodology for estimation of cerebrospinal fluid (CSF) tracer clearance could have wide clinical application in predicting excretion intrathecal drugs and metabolic solutes from brain metabolism diagnostic workup CSF disturbances.
Abstract Microfluidic technology is a valuable tool for realizing more in vitro models capturing cellular and organ level responses rapid animal‐free risk assessment of new chemicals drugs. cell‐based devices allow high‐throughput screening flexible automation while lowering costs reagent consumption due to their miniaturization. There growing need faster approaches drug development safety (Registration, Evaluation, Authorisation Restriction Chemical Substances, REACH). The work presented...
Quantitative measurements of cerebrospinal fluid to blood clearance has previously not been established for neurological diseases. Possibly, variability in may affect the underlying disease process and possibly be a source under- or over-dosage intrathecally administered drugs. The aim this study was characterize magnetic resonance imaging contrast agent gadobutrol (Gadovist, Bayer Pharma AG, GE). For this, we population pharmacokinetic model, hypothesizing that differs between Gadobutrol...
3D cell culture models exposed at the air-liquid interface (ALI) represent a potential alternative to animal experiments for hazard and risk assessment of inhaled compounds. This study compares cocultures composed either Calu-3, A549 or HBEC3-KT lung epithelial cells, cultured together with THP-1-derived macrophages EA.hy926 endothelial in terms barrier capacity responses standard reference sample fine particulate matter (SRM 2786). High-content imaging analysis revealed similar cellular...
Polybrominated diphenyl ethers (PBDEs) are widely used brominated flame retardants (BFRs), which have become ubiquitous in the environment. This study investigates effects of pentabrominated ether mixture, DE-71, on human neutrophil granulocytes vitro. DE-71 enhanced production reactive oxygen species (ROS) a concentration-dependent manner measured as lucigenin-amplified chemiluminescence. Octabrominated (OBDE), decabrominated (DBDE), and non-brominated did not induce ROS formation at...
Studies of synapsin-deficient mice have shown decreases in the number synaptic vesicles but knowledge about consequences this decrease, and which classes are being affected, has been lacking. In study, glutamatergic, GABAergic dopaminergic transport analysed animals where genes encoding synapsin I II were inactivated. The levels vesicular glutamate transporter (VGLUT) 1, VGLUT2 GABA (VGAT) decreased by approximately 40% adult forebrain from devoid II, while monoamine (VMAT) 2 VGLUT3 present...